High-Throughput Mutagenesis and Screening of Phage Libraries with Altered Biological Functions

This invention presents novel synthetic bacteriophages and compositions, offering methods for their production and therapeutic uses. With the escalating challenge of drug-resistant bacterial infections, these synthetic bacteriophages address limitations in antibiotic research by providing an alternative therapy approach. By engineering phages with altered host ranges, the technology allows for the rapid evolution of combinatorial phage libraries, surpassing the limitations of natural phage evolution and offering a powerful solution for treating bacterial infections and delaying the evolution of bacterial strains.