A Ribonucleoprotein Approach to Boost the STING Signaling for Cancer Immunotherapy

Disclosed herein is a non-covalent complex, comprising; a tetramer of a recombinant protein; and an agonist of a Stimulator of Interferon Gene (STING) protein or a pharmaceutically acceptable salt thereof, wherein the recombinant protein comprises a STING protein lacking a transmembrane domain (STINGΔTM protein). Additionally, provided is a vaccine composition, comprising a non-covalent complex and a pharmaceutically acceptable carrier, wherein the non-covalent complex comprises: a recombinant protein comprising a STINGΔTM protein and a tumor epitope; and an agonist of a STING protein or a pharmaceutically acceptable salt thereof. Further provided are methods of treating and preventing cancer using the disclosed complexes, pharmaceutical compositions, and vaccines.