Stochastic Barcoding Method for Single-Cell Tracking

Exclusively Licensed

A method of tracking cell identity across analytical platforms uses stochastic barcoding (SB). SB uses a randomly generated code based on one or more of the number, color and position of beads encapsulated together with a set of cells of interest. SB use is demonstrated in an application where cells are transferred from a microwell array into a microtiter plate while keeping their identity, and obtained an average identification accuracy of 96% for transfer of 100 blocks. Model scaling of the method up to 1000 blocks demonstrated that SB is able to achieve approximately 90% accuracy.

Researchers

Darrell J Irvine / Marc Castellarnau / Gregory Szeto / J. Christopher Love / Joel Voldman

Departments: Department of Chemical Engineering, Dept of Electrical Engineering & Computer Science
Technology Areas: Biotechnology: DNA & RNA Editing, Synthetic Biology / Drug Discovery and Research Tools: Cell Interrogation, Genomics & Proteomics
Impact Areas: Healthy Living

  • method and apparatus for tracking cell identity
    United States of America | Granted | 9,709,479

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