Single-Cell RNA Sequencing Using Click-Chemistry
Invention type: Technology
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Case number: #20396
The present disclosure relates to a method of sequencing nascent RNA in a cell. In some embodiments, the nascent RNA is conjugated to DNA using copper-catalyzed azide- alkyne cycloaddition (CuAAC). Methods of the present disclosure can be used to generate genomic libraries of a cell and measure gene expression and enhancer and/or super-enhancer activity.
Researchers
Departments: David H Koch Institute for Integrative Cancer Res, Office of the Institute Professors
Technology Areas: Biotechnology: DNA & RNA Editing / Chemicals & Materials: Catalysis & Synthesis / Drug Discovery and Research Tools: Cell Interrogation, Genomics & Proteomics / Therapeutics: Cell Based Therapy
Impact Areas: Healthy Living
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single-cell rna sequencing using click-chemistry
United States of America | Granted | 11,519,027 -
single-cell rna sequencing using click-chemistry
United States of America | Published application
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