Selective Bfl-1 Peptides


Peptide inhibitors of Bfl-1, an anti-apoptotic protein, allow for selective targeting of Bfl-1 dependent cancer cells in therapeutic treatment. Selective peptides may also be used as Bfl-1-detection reagents. This allows for cellular profiling for anti-apoptotic protein dependency, thus potentially acting as a diagnostic tool for stratifying patients. 

Problem Addressed

Many cancer cells overexpress anti-apoptotic Bcl-2 family proteins that confer resistance to chemotherapy treatment. Selective inhibitors of Bcl-2 are available in clinic to treat Bcl-2 dependent cancers. However, such tools are missing for Bfl-1. Selective inhibitors allow for selective targeting of Bfl-1 dependent cancer cells while limiting systemic toxicity.


Selective peptide inhibitors of Bfl-1 were identified by screening for sequence variants of the BH3 motif of PUMA, a highly potent binder of Bcl-2 family members. Peptides were designed using computational analysis followed by experimental screening that identified peptides with high affinity for Bfl-1 yet weakened interactions with other anti-apoptotic proteins. The lead peptide inhibitors are >100-fold selective for binding to Bfl-1 binding over other anti-apoptotic proteins of Bcl-2 family. Selectivity towards Bfl-1 was further augmented by modifying the peptide inhibitors with electrophiles to allow for covalent interaction in the binding pocket of Bfl-1. The selectivity of the peptide Bfl-1 inhibitors in a cellular context was confirmed by the relative mitochondrial depolarization or cytochrome-c release after peptide treatment of Bfl-1 dependent cells vs. other anti-apoptotic protein dependent cells.


  • The peptide inhibitors are potent and selective towards Bfl-1 with >100-fold selectivity over other anti-apoptotic proteins
  • The peptide inhibitors can also be used as diagnostic tools to profile cells for Bfl-1 dependency