In Situ Sequencing of Expanded Biological Specimens

Non-Exclusively Licensed

The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids as well as new methods for fluorescent in situ sequencing (FISSEQ) in a new process referred to herein as “expansion sequencing” (ExSEQ).

Researchers

Edward Stuart Boyden / Shahar Alon / Evan Daugharthy / Adam Marblestone / Paul Tillberg / Fei Chen / George Church

Departments: Department of Brain and Cognitive Sciences, Health Sciences and Technology Program
Technology Areas: Biotechnology: Biomedical Devices & Systems, Sensors & Monitoring / Drug Discovery and Research Tools: Genomics & Proteomics / Sensing & Imaging: Imaging
Impact Areas: Healthy Living

  • in situ nucleic acid sequencing of expanded biological samples
    United States of America | Granted | 10,059,990
  • in situ nucleic acid sequencing of expanded biological samples
    United States of America | Granted | 10,563,257

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