The Site-Specific Incorporation of a Redox-Active and Chemically-Reactive Amino Acid into Proteins in E. Coli

This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.

Researchers

Peter Schultz / Jianming Xie / Mohammad Seyedsayamdost / Clement Tsz Chan / Lital Alfonta / JoAnne Stubbe

Departments: Department of Chemistry
Technology Areas: Therapeutics: Nucleic Acids, Proteins & Antibodies
Impact Areas: Healthy Living

  • genetic incorporation of 3-aminotyrosine into reductases
    United States of America | Granted | 8,673,591

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