Method for Screening Libraries of Enzymes with Improved Catalytic Properties or Altered Substrate Specificity Using Microwells for the Spatial Separation of Cells Producing the Enzymes

Exclusively Licensed

The invention provides a method for isolating particular members from a library of variant cells in individual microreactors, wherein the phenotype of the biomolecule secreted by the cell is evaluated on the basis of multiple parameters, including substrate specificity and kinetic efficiency.

Researchers

Kerry Love / J. Christopher Love

Departments: Department of Chemical Engineering
Technology Areas: Biotechnology: Biomanufacturing, Synthetic Biology / Chemicals & Materials: Catalysis & Synthesis / Diagnostics: Assays / Therapeutics: Proteins & Antibodies
Impact Areas: Healthy Living

  • method for screening libraries of enzymes
    Germany | Granted | 2,297,333
  • compositions and methods for spatial separation and screening of cells
    Canada | Granted | 2,763,790
  • method for screening libraries of enzymes
    European Patent Convention | Granted | 2,297,333
  • method for screening libraries of enzymes
    Ireland | Granted | 2,297,333
  • method for screening libraries of enzymes
    Liechtenstein | Granted | 2,297,333
  • method for screening libraries of enzymes
    United Kingdom | Granted | 2,297,333

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