Kinase Sensing via Hydroxyquinoline-Sensitized Europium Chelates

Exclusively Licensed

The present invention generally relates to assays of enzymes responsible for phosphoregulation including kinases (such as protein kinases, which mediate protein and peptide phosphorylation) and phosphatases (such as protein phosphatases, which mediate protein and peptide dephosphorylation). Certain aspects of the invention use lanthanide ions such as europium ions that exhibit chelation-enhanced luminescence. Phosphorylation of a peptide by a kinase may cause a complex to form between the lanthanide ion, the phosphate group, and a reporter group such as a hydroxyquinoline, which results in luminescence when in the complexed state. Thus, in certain embodiments, determination of luminescence may be indicative of kinase activity. Certain embodiments also include the use of substrates for detection of phosphatase activity, where dephosphorylation results in a loss of signal assay. Other aspects are generally related to techniques for making and using such peptides or complexes, kits involving such peptides or complexes, and the like.

Researchers

Barbara Imperiali / Juan Gonzalez Vera

Departments: Department of Biology
Technology Areas: Diagnostics: Assays / Sensing & Imaging: Chemical & Radiation Sensing, Imaging / Therapeutics: Proteins & Antibodies
Impact Areas: Healthy Living

  • kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
    Netherlands | Granted | 3,765,629
  • kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
    United States of America | Granted | 10,799,603
  • kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
    Japan | Granted | 7,337,141
  • kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
    China | Granted | 112,105,742
  • kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
    European Patent Convention | Granted | 3,765,629
  • kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
    Germany | Granted | 3,765,629
  • kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
    France | Granted | 3,765,629
  • kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
    United Kingdom | Granted | 3,765,629

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