Kinase Sensing via Hydroxyquinoline-Sensitized Europium Chelates
The present invention generally relates to assays of enzymes responsible for phosphoregulation including kinases (such as protein kinases, which mediate protein and peptide phosphorylation) and phosphatases (such as protein phosphatases, which mediate protein and peptide dephosphorylation). Certain aspects of the invention use lanthanide ions such as europium ions that exhibit chelation-enhanced luminescence. Phosphorylation of a peptide by a kinase may cause a complex to form between the lanthanide ion, the phosphate group, and a reporter group such as a hydroxyquinoline, which results in luminescence when in the complexed state. Thus, in certain embodiments, determination of luminescence may be indicative of kinase activity. Certain embodiments also include the use of substrates for detection of phosphatase activity, where dephosphorylation results in a loss of signal assay. Other aspects are generally related to techniques for making and using such peptides or complexes, kits involving such peptides or complexes, and the like.
Researchers
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kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
Netherlands | Granted | 3,765,629 -
kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
United States of America | Granted | 10,799,603 -
kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
Japan | Granted | 7,337,141 -
kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
China | Granted | 112,105,742 -
kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
European Patent Convention | Granted | 3,765,629 -
kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
Germany | Granted | 3,765,629 -
kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
France | Granted | 3,765,629 -
kinase and/or phosphatase sensing via hydroxyquinoline-sensitized chelates
United Kingdom | Granted | 3,765,629
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