An Integrated and Automated Electronic System for Point-of-Care Protein Testing

Microfluidic chips containing electrochemical biosensors are described. The electrochemical biosensors include a flow layer intersected by valves of a control layer, which control the fluid flow. The flow layer includes two zones, an analyte capture zone for mixing a sample with an analyte capture element, and a detection zone for detecting the analyte. Both zones include a rotary mixer for mixing, and where needed, trapping, washing, and flowing the captured analyte. The captured analyte is detected by the sensing region of the detection zone. The microfluidic chips may be integrated into devices for automated, fast, point-of-care determination of analyte concentration.