High-throughput Electroporation Device

Technology

This electroporation system works by directing the flow of cell samples to a collection unit for highthroughput processing. The configuration, working with a liquid handling system, features a minimum of two electrically conductive layer components, with a removable channel layer component positioned in between. The electrically conductive layers and channel layers establish the parallel electroporation flow paths extending from the top to the base of the configuration and cell collection unit. Along the flow paths, the conductive layers generate electric fields of various sizes and intensities through which cells pass. Electrically insulating support layers run parallel to the electrically conductive layers, configuring the inputs and outputs for cell travel. Inputs receive cell sample fluid from a manual pipette, automated liquid dispenser, and/or an automated liquid handling system, while the outputs direct the cells into the cell collection unit.

Problem Addressed

The effective delivery of small molecules to cells is a critical step in many biological processes. Among other strategies, electroporation stands out as the preferred method due to its ability to deliver both small and large molecules to any cell type with relatively high efficiency. During electroporation, cells are briefly exposed to an electrical current which creates temporary pores along the cell membrane through which molecules can enter.

Current electroporation methods require extensive manual pipetting, provide medium cell throughput, and are limited to mammalian cells. This technology overcomes these limitations by minimizing human error through automated pipetting, incorporating scalable assembly parameters, and utilizing continuous flow-based liquid handling systems to generate a high-throughput electroporation assembly.

Advantages

• This assembly provides a scalable, high-throughput electroporation platform compatible with commercially available liquid handling robots for automated pipetting.
• This device can be used with various liquid handling and liquid collection systems.
• The layered configuration allows the device to be readily fabricated using standard, widely available manufacturing techniques.
• Flow-through based electroporation improves cell viability and reduces the rate of arcing events.

Publications

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