Fully-synthetic Extracellular Microenvironments for in Vitro Stem Cell Propagation and Organogenesis

Non-Exclusively Licensed

Synthetic hydrogels for organogenesis support organogenesis from mammalian cells, including human cells. The synthetic hydrogels typically include a network of crosslinked branched biodegradable polymers. A portion of the branches of the branched biodegradable polymers are linked to binders which are generally synthetic peptides for cell and extracellular matrix attachment. The hydrogels may include an inhibitor of apoptosis. The synthetic hydrogels with the synthetic binders typically do not interfere with cellular, proteomic, genetic, and/or transcriptome analyses of organoids formed in the hydrogel. The synthetic hydrogels may be subject to on-demand dissolution to provide intact organoids substantially free of hydrogel polymers. Also provided are methods of making the synthetic hydrogels and methods of using the synthetic hydrogels for organogenesis.

Researchers

Linda Griffith / Victor Hernandez-Gordillo

Departments: Department of Biological Engineering
Technology Areas: Chemicals & Materials: Polymers / Drug Discovery and Research Tools: Cell Culture
Impact Areas: Healthy Living

  • synthetic hydrogels for organogenesis
    European Patent Convention | Published application
  • synthetic hydrogels for organogenesis
    United States of America | Granted | 12,084,685
  • fully-synthetic extracellular microenvironments for in vitro stem cell propagation and organogenesis
    United States of America | Pending
  • synthetic hydrogels for organogenesis
    China | Published application
  • synthetic hydrogels for organogenesis
    Canada | Published application

Publications

Victor Hernandez-Gordillo, Timothy Kassis, Arinola Lampejo, GiHun Choi, Mario E. Gamboa, Juan S. Gnecco, Alexander Brown, David T. Breault, Rebecca Carrier, and Linda G. Griffith. "Fully Synthetic Matrices for In Vitro Culture of Primary Human Intestinal Enteroids and Endometrial Organoids." Biomaterials 254 (2020): 120125. https://doi.org/10.1016/j.biomaterials.2020.120125.

License this technology

Interested in this technology? Connect with our experienced licensing team to initiate the process.

Sign up for technology updates

Sign up now to receive the latest updates on cutting-edge technologies and innovations.

More Technologies