Barcoded Combinatorial Genetic Screening with CRISPR-CAS

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Described herein are methods and compositions that enable rapid generation of high-order combinations of genetic elements comprising a CRISPR guide sequence and a scaffold sequence, and a barcode for rapid identification of the combination of genetic elements encoded within a single cell or a pooled population. Also described herein compositions of inhibitors of epigenetic genes and methods for reducing cell proliferation and/or treating cancer.

Researchers

Timothy K Lu / Ching Gee (Gigi) Choi / Alan Siu Lun Wong

Departments: Department of Biological Engineering
Technology Areas: Biotechnology: Biomanufacturing, DNA & RNA Editing / Therapeutics: Cell Based Therapy, Nucleic Acids
Impact Areas: Healthy Living

  • massively parallel combinatorial genetics for crispr
    United States of America | Published application
  • massively parallel combinatorial genetics for crispr
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    China | Granted | 0
  • massively parallel combinatorial genetics for crispr
    Japan | Granted | 6,788,584
  • massively parallel combinatorial genetics for crispr
    European Patent Convention | Granted | 3,212,789
  • massively parallel combinatorial genetics for crispr
    Switzerland | Granted | 3,212,789
  • massively parallel combinatorial genetics for crispr
    Germany | Granted | 3,212,789
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    France | Granted | 3,212,789
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    United Kingdom | Granted | 3,212,789
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    Liechtenstein | Granted | 3,212,789
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    Italy | Granted | 3,212,789
  • massively parallel combinatorial genetics for crispr
    Netherlands | Granted | 3,212,789

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