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Problem Addressed

The COC devices address the many problems inherent to the use of microfluidic devices made of polydimethylsiloxane (PDMS), such as incompatibility with organic solvents, low mechanical strength, and tendency to absorb small molecules. Typical bonding methods for joining COC materials with each other or with glass and poly(methyl methacrylate) (PMMA) often involve flammable, toxic chemicals, or high temperatures. Sometimes the bonding material leaks into the channels of the microfluidic device, or a channel collapses, leading to a blockage and defective device. The use of toxic chemicals also runs the risk of leaving residue behind that can negatively affect one’s cellular experiments or interfere with other chemical reactions in the device. Some of the current manufacturing methods with COC also require specialized equipment. However, the methods described in this patent avoid the above disadvantages.