Header and Body 3


This bacterial lysis mechanism generates ICP near the ion selective membranes of the bacteria to shear and electrically permeate the bacteria. This enables the use of low voltage electric fields (100-300 V/cm) in common buffers such as phosphate to lyse the bacteria. This technique facilitates the recovery of intracellular biomaterials such as proteins and RNA’s from both easy-to-lyse and hard-to-lyse bacteria that previously required low salinity solutions and extremely high electric fields (4000-8000 V/cm). This technology can be adjusted to lyse bacteria at large scales by combining multiple lysis mechanisms into a laterally-arrayed electromechanical lysis device.