This technology uses an artificial intelligence neural network to identify the lab of origin of an engineered DNA sequence. The design choices made when building a new DNA tool are frequently dictated by lab history and culture, which leads to sequence similarities in DNA arising from any given laboratory. In addition to these rational design choices, unintentional marks such as silent mutations that don’t affect DNA functionality can create a unique signature for DNA constructs synthesized in a particular lab. This technology uses machine learning and artificial intelligence software to identify DNA sequence signatures that identify the lab-of-origin of the DNA. The inventors demonstrate that this AI technique is superior to BLAST, the most common DNA sequence comparison technique, at identifying lab-of-origin for engineered DNA, particularly for short sequences with commonly used motifs.