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Technology

This invention consists of a system comprising: (1) A repressor protein (RP) that is either produced within a cell or added to a cell-free biological system; (2) A regulatory RNA sequence that when added in single or multiple copy to the 5' or 3' untranslated region (UTR) of a gene encoding the target protein, is capable of forming an RNA structure (aptamer) that binds the RP (1) with high affinity in the context of a cellular environment or a cell-free system; and (3) a regulatory molecule capable of interacting with the RP and altering its interaction with the aptamer (2).