Header and Body 3


This technology describes a method of metabolic engineering for the microbial production of branched, medium chain alcohols. The proposed metabolic pathway is composed of enzymes from multiple organisms and pathways, recombinantly expressed in E. coli. In one iteration of the process, two precursors (isobutyryl-CoA and acetyl-CoA) are generated from glycolysis, condensed to form a branched intermediate, and reduced to the final product, 4MP, in six steps. The pathway can be modulated based on enzyme expression to produce a variety of branched, medium chain alcohols.