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Applications

FLIP cassette-containing plasmid vectors (pFLIP), which is suitable for lentiviral transgenesis, can be used  to transduce Cre mice and cells, creating constitutive and inducible lines expressing miR30-based RNAi constructs that knock down a gene of interest. This targeted knockdown is commonly used to investigate the role of particular genes in development and disease progression. The FLIP cassette can also contain reporter genes such as Thy1.1, eGFP, or RFP to test for viral titer.