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Problem Addressed

Many proteome analysis technologies, such as array tomography, that are used to label and visualize 3D pieces of tissue do not allow for the joint measurement of many molecular and anatomical traits in the same piece of tissue, or are difficult to implement in most labs. In recent years, tissue-clearing techniques, such as CLARITY, have enabled scientists to pass each piece of tissue through a few rounds of labeling, in order to visualize the interactions between different cell types and molecules in the same sample. However, the tissue loses structural integrity upon repeated rounds of relabeling. A simple tissue-clearing technique that preserves the structural integrity of tissue after many rounds of relabeling will greatly improve upon current 3D tissue imaging techniques.