Header and Body 1


The generation of robust, scalable gene networks that interfere minimally with endogenous cellular processes is fundamental to synthetic biology. The use of RNA regulatory strategies in combination with CRISPR/Cas transcription factors (CRISPR-TFs) allows for the conditional regulation of large-scale synthetic and endogenous gene networks in eukaryotic cells. The system is uniquely suited to build sophisticated circuits and transcriptional cascades that could be linked to specific events (e.g., cell cycle) or environments (e.g., chemical gradients).