Matrix metalloproteases (MMPs) are enzymatic proteins that are important for many physiological roles in development and adulthood. MMPs are an attractive diagnostic and therapeutic target because MMP expression and activity is dysregulated in many diseases including cancer, cardiovascular disease, endometriosis, pre-eclampsia, and arthritis. However, detection of MMP activity remains a challenge. Current technologies often fail to differentiate between active and inactive forms, require large sample sizes, and only detect single MMPs at a time. This technology is a simple, multiplexed solution to detect MMP activity.